Our aim was to determine the quantitative effects of a single-dose of Nattokinase (NK) administration on coagulation/fibrinolysis parameters comprehensively in healthy male subjects.
A double-blind, placebo-controlled cross-over NK intervention study was carried out in 12 healthy young males. Following the baseline blood draw, each subject was randomized to receive either a single-dose of 2,000 FU NK (NSK-SD, Japan Bio Science Laboratory Co., Ltd) or placebo with subsequent cross-over of the groups. Subjects donated blood samples at 2, 4, 6 and 8 hours following administration for analysis of coagulation/fibrinolysis parameters.
As a result, D-dimer concentrations at 6, and 8 hours, and blood fibrin/fibrinogen degradation products at 4 hours after NK administration elevated significantly (p < 0.05, respectively). Factor VIII activity declined at 4 and 6 hours (p < 0.05, respectively), blood antithrombin concentration was higher at 2 and 4 hours (p < 0.05, respectively), and the activated partial thromboplastin time prolonged significantly at 2 and 4 hours following NK administration (p < 0.05 and p < 0.01, respectively). All the changes, however, were within the normal range.
In conclusion, thus, a single-dose of NK administration appears enhancing fibrinolysis and anti-coagulation via several different pathways simultaneously.
Department of Sport and Health Science, Ritsumeikan University, Kusatsu, Shiga, Japan
- Yuko Kurosawa, Shinsuke Nirengi, Toshiyuki Homma & Takafumi Hamaoka
Department of Economics, Tokuyama University, Shunan, Yamaguchi, Japan
- Kazuki Esaki
Department of Clinical Chemistry, Kobe Pharmaceutical University, Kobe, Hyogo, Japan
- Mitsuhiro Ohta
Department of Neurology, University of Cincinnati, Cincinnati, Ohio, USA
- Joseph F. Clark
Scientific Reports 5, Article number: 11601 (2015) doi:10.1038/srep11601